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Analysis of MAb Fc Glycans Using an RPL Biosensor

Detection of Glycoproteins & Glycoprotein Glycoforms Using an RPL Biosensor

RPL functionalised biosensor platforms present exciting opportunities as enhanced glycoanalytical tools enabling simple, rapid, real time, label free detection, analysis and quantification of glycoproteins with high sensitivity and using small sample volumes. Use of high throughput paltforms could facilitate applications in biopharmaceutical product development or their use as process analytical tools to enhance the fidelity and efficiency of production processes.

Biosensor Based Analytical Platforms

GlycoSeLects RPLs can be readily immobilized onto a wide range of analytical platforms, using standard chemistries and approaches (covalent or affinity based immobilization), to create glycoanalytical tools for specific end user applications.

"Biochip" by Argonne Laboratory's Flickr page 

RPL Functionalised Analytical Platforms

Lectin arrays enable simple, fast and high-throughput analysis of intact glycosylated biomolecules including whole cells. Analysis of intact biomolecules results in the acquisition of more physiologically relevant information that is often lost in more traditional methods of glycoanalysis which require the prior release of glycans. They have found widespread applications including use for the analysis of biopharmaceutical products, biomarker discovery and diagnostics.

Lectin Arrays & Biochips

ForteBio: Biolayer Interferometry (BLI) biosensor tips (top left) for the detection and  analysis of biomolecular interactions. The Octet Red platform (top right) enables high throughput applications.

Due to their prokaryotic origins RPLs do not contain any disufide bonds enabling analysis to be performed under denaturing conditions. This can enable simplified analysis of glycoproteins, such as MAbs, were glycans may be presented in sterically restricted locations.

Biosensor based platforms can be used for a broad spectrum of applications across the life sciences including applications in fundamental research, biopharmaceutical analysis and characterisation and diagnostics.