using RPL Functionalised Bioaffinity Matrices
Achieve Efficient Isolation of IgM from Complex Media
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RPL production can be simply and cost effectively scaled to produce them in sufficient quantities required to enable their use for large scale applications. This would would include preparative scale applications, applications in process development or ultimately manufacturing scale purification of glycosylated biomolecules.
GlycoSeLective Purification Tools for all Application Scales
RPL-Man 2 Sepharose Column
RPL-Gal1 Sepharose Column
RPLs can be immobilized onto solid support matrices using standard chemistries and approaches (covalent & bioaffinity based). They can be immobilized at high densities (up to 20mg per mL of resin) generating high binding capacity biaffinity matrices.
RPL bioaffinity matrices exhibit excellent stability and reproducibility in successive runs. Because of their prokaryotic origins RPLs lack disulphide bonds making them compatible with reducing agents for applications where denaturing conditions are required.
High Capacticity & Robust Bioaffinity Matrices
Right Panel: Separation of a mixture of Human Transferrin and asialo-Transferrin glycoforms (1mg of each glycoform) using a 1mL RPL-Gal1 Sepharose column.
RPL Functionalised Magnetic
Separation of Transferrin & Asialotransferrin Glycoforms
Left Panel: Isolation of asialotransferrin (AsT) from a mixture (M) of GFP (G - nonglycosylated) and RNAseB (R - high mannose) using RPL-Gal1 functionalised mag beads. AsT was clearly absent from the unbound fraction (U) and was recovered in the galactose eluted fraction (E). Use of RPL magnetic particles is readily automated for smale scale high throughput isolation of glycoproteins.
RPLs can be immobilizes onto a wide range of different support matrices, including magnetic particles, sepharose and monolithic supports, to produce highly efficient glycoselective separation and purification tools for specific end user needs. Samples can be applied in physiological buffers, or from complex media, and bound products are efficiently recovered through simple incorporation of an appropriate sugar ligand into elution buffers. Samples are not exposed to harsh conditions (salt, pH or solvents) and are recovered in their native form for downstream applications.
RPL Based Bioaffinity Purification Tools