Because glycosylation is not a template driven processes the nature of the glycans displayed on a biomolecule cannot be easily predicted. This imposes an absolute requirement for the isolation of glycosylated biomolecules in order to enable their characterisation and to identify the glycans present. Traditionally this has been a significant technical challenge and there is a need for new approaches and tools to facilitate more efficient isolation and analysis of glycosylated biomolecules. Lectins are powerful tools that can meet these needs.
The Need for Novel Tools for the Glycosciences
In 1990, the University of Oxford quoted "GlycoScience is the fastest growing science in the medical world." In 2003, MIT said "GlycoScience is one of the 10 emerging technologies that will change the world."
The Biological Roles of Glycans
Glycoscience, the study of glycans and glycosylated biomolecules, is the next frontier in medical science.
Unlike other biological processes, such as DNA and protein biosynthesis, glycosylation is not a template driven processes. The glycans that are ultimately displayed on a biological molecule are the result of the complex series of enzymatic processes. The glycans displayed on biomolecules and cell surfaces vary depending on the source organism or cell type, as a result of changes in physiological status of a cell or environmental conditions, or as a result of the onset/remission of disease.
Erythropoietin (EPO):A glycosylated glycoprotein based biotherapeutic displaying three complex N-linked glycans (red) and one O-linked glycan (orange) known to impact on its efficacy. Image Courtesy of Pauline Rudd & NIBRT
The glycans displayed by biomolecules can mediate a vast and diverse range of biological processes, often through interaction with carbohydrate binding proteins called Lectins. This includes roles in cell signaling, cell migration and orchestration of the the immune system. They also mediate interactions with infectious agents such as prions, viruses and microorganisms. Changes in the glycans displayed can also be the cause, or the result, of diseases including many types of cancers. Glycans can also have a significant impact on the functionality and properties of the biological molecules to which they are attached. This is of particular relevance to the Biopharmaceutical industry where the majority of biotherapeutic molecules in production, and development, are glycoproteins and the glycans displayed can affect their efficacy and safety. Identifying and studying the glycan structures present on glycosylated biomolecules is therefore and essential step in understanding this important class of biological molecules and in helping to decipher complex biological processes.
Glycoscience - The New Frontier In Bioscience
Glycosylation & Glycans
Glycosylation is a biological process by which complex carbohydrate structures, called glycans, are attached to biological molecules such as lipids (glycolipids) and proteins (glycoproteins).