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RPL-Gal2 exhibits approximately 5 fold higher affinity than GSL-I

RPL-Gal1 exhibits approximately 10 fold higher affinity than ECL

ELLA Detection of

Terminal Alpha-Linked Galactose

ELLA Detection of

Terminal Beta-Linked Galactose

Higher Sensitivity: In standard ELLA formats RPLs enable significantly greater sensitivity of detection at lower lectin concentrations than that required for plant lectins

Higher Affinity: RPLs exhibit greater selectivity and significantly higher affinity for glycosylated targets than comparable plant lectins.

Direct Detection Without The Need for In Vitro Labeling

Enhanced Selectivity, Affinity & Sensitivity of Detection

These simple assay formats are widely used across the Life Sciences for a broad spectrum of applications.

RPLs are expressed with genetically incorporated poly-histidine tags. This not only simplify their purification but also enables their detection without the need for in vitro labeling, such as biotinylation, that is typically required for detection of plant and other eukaryotic lectins. RPLs can be simply detected through the use of standard commercially available conjugated/labeled anti-his antibodies.

-  Enzyme Linked Lectin Assays (ELLAs)      PubMed

-  Lectin (Western) Blotting

-  Histochemical Staining 

RPLs can be used as reagents for the detection and analysis of intact glycosylated biomolecules in a wide range of traditional experimental formats:

Enzyme Linked Lectin Assay (ELLA)

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RPLs - Biochemical Assay Reagents